Characterization of <i>Hoplolaimus seinhorsti</i> and <i>Hoplolaimus pararobustus</i> (Tylenchina: Hoplolaimidae) from banana, with phylogeny and species delineation in the genus <i>Hoplolaimus</i>

Abstract The morphological and molecular characterisations of two lance nematode species isolated from the rhizosphere banana, Hoplolaimus seinhorsti H. pararobustus , are provided based on an integrative study that includes light scanning electron microscopy, phylogenetic analysis tree-based delimitation methods (GMYC bPTP). Nineteen new sequences were obtained, including 5 partial 18S rRNA, 6 D2-D3 28S 1 ITS rRNA 7 COI mtDNA (the first ), updated character comparison 37 is presented. species-delimitation approaches employed gave markedly differing results, also showed remarkable discrepancies among investigated genes, although bPTP output was found to agree well with established delimitations. Both did, however, provide same for sequences, gene sequence correspond better species. It therefore recommended by this paper as representing most suitable barcode marker identification. This resulted in corrective reassignment 17 previously unidentified or incorrectly classified, concluding consists cryptic